| Cell
Line Name |
KCL003/CF-1 |
| Cell Line Status |
Currently available |
| Cell Type |
Human Embryonic |
| Grade |
Research |
| Source |
Human embryo / blastocyst |
| Source type |
Cryopreserved |
| Consent Status |
Donor consent was reviewed by the UK Steering Committee and was
found to be consistent with the Steering Committee Code of Practice
for the Use of Human Stem Cell Lines. |
| Date cell line derived |
01 May 2003 |
| Ethical Statement |
In the opinion of the Steering Committee for the UK Stem Cell
Bank and the use of Human Stem Cell Lines, the derivation of the
stem cell line is consistent with UK ethical guidelines |
| Depositor |
Kings College London |
| Depositing Centre |
Kings College, London |
| Country of Origin |
United Kingdom |
| Depositor Contact
Details |
cecilia.soh@kcl.ac.uk |
| Journal Reference |
Stem cell line encoding the cystic fibrosis mutation - SJ
Pickering et al. Vol 10. No 3. 2005 390-397 Reproductive
BioMedicine |
| SCSC No. |
SCSC-06-28(2) |
| UKSCB Accession No. |
R-08-030 |
| Distribution Bank No. |
d01 |
| Passage No. |
p19 |
| Feeder Cell Type |
Mouse embryo fibroblast |
| Basel Medium |
DMEM |
| Protein Source |
FBS |
| Growth Factors |
bFGF and LIF |
| Incubation Conditions |
37°C, 5% CO2, >95% RH, Subculture: manual cutting
|
| Culture Morphology |
Embryonic stem cell colonial morphology |
| Preservation Method |
Vitrified |